FSP - Scientist - Transient Expression

Work ScheduleStandard (Mon-Fri)Environmental ConditionsLaboratory Setting, Some degree of PPE (Personal Protective Equipment) required (safety glasses, gowning, gloves, lab coat, ear plugs etc.)Job DescriptionThis is a fully onsite role based at our customer’s site in San Diego, CA. We welcome applicants from all locations within the US. Please note that relocation assistance is not provided for this position, and any relocation costs will be the responsibility of the candidate.  Must be legally authorized to work in the United States without sponsorship. Must be able to pass a comprehensive background check, which includes a drug screening. At Thermo Fisher Scientific, you’ll discover meaningful work that makes a positive impact on a global scale. Join our colleagues in bringing our Mission to life - enabling our customers to make the world healthier, cleaner and safer. We provide our teams with the resources needed to achieve individual career goals while taking science a step beyond through research, development and delivery of life-changing therapies. With clinical trials conducted in 100+ countries and ongoing development of novel frameworks for clinical research through our PPD clinical research portfolio, our work spans laboratory, digital and decentralized clinical trial services. Your determination to deliver quality and accuracy will improve health outcomes that people and communities depend on – now and in the future.Thermo Fisher's clinical research business is a leading global contract research organization and world leader in serving science. We are passionate, deliberate, and driven by our mission – to enable our customers to make the world healthier, safer, and cleaner.Our Laboratories Functional Service Partnership (FSP) team plays a direct role in advancing drug development by creating sustained groups of talent within our customers’ organizations to provide long-term support. Through this collaborative approach, our professionals bring their commitment to excellence, industry knowledge, and operational strength directly into customer environments, helping to enhance capabilities and advance the delivery of life-changing therapies.Key responsibilities:The role encompasses the production of monoclonal antibodies and other proteins via molecular cloning, DNA preparation, cell line maintenance, transient transfection of production cell lines, and stable cell line production. Some experience with AAV expression is also desirable but not required. The candidate is expected to design and perform studies while also working collaboratively with colleagues across the R&D organization to support the development of biological therapeutics. The ideal candidate will have hands-on experience in producing proteins from mammalian cell culture, preferably in a biotechnology or pharmaceutical company setting.Assist with cell culture, transient transfection, expression optimization, DNA preparation, and protein quantificationCulture mammalian cell lines for DNA transfection with rigorous sterile techniqueExplore potential improvements to the existing protein expression platform under the direction of a senior scientist.Optimize experimental procedures drawing on available literature and previous resultsPartner in the development of a stable cell line expression platform for research purposesWork with bioreactors for transient and stable CHO expressionPrepare detailed data reports and presents results to peers and colleagues.Other duties as assigned.Education and Experience: Bachelor's degree in lab sciences such as Chemistry, Biochemistry, Material Science, Immunology, Biology, Molecular Biology or similarScientist: Previous experience that provides the knowledge, skills, and abilities to perform the job (comparable to 2+ years’)OR Masters degreeIn some cases, an equivalency, consisting of a combination of appropriate education, training and/or directly related experience, will be considered sufficient for an individual to meet the requirements of the role.Knowledge, Skills and Abilities: Experience with cell culture and transient transfection in CHO and HEK293 cellsExperience with antibody expression/productionKnowledge of stable cell line developmentKnowledge of protein expression using a bioreactorExperience with AAV expression would be highly desirableKnowledge of standard molecular biology cloning techniques is a plusAble to execute experiments efficiently and suggest small process improvementsRecognizes fundamental anomalies in data points and identifies issues in experiments / processesBegins to understand how to think outside of the technical process and consider the impact decisions will have on the broader scientific goalsStrong knowledge of scientific disciplineGood knowledge of scientific principles, methods and techniquesGood knowledge and demonstrated ability working with a variety of laboratory equipment/toolsStrong computer skillsGood problem-solving, analytical thinking skillsDetail orientedAbility to meet deadlinesExcellent communication skills with the ability to collaborate with cross-functional scientistsAbility to maintain accurate detailed documentation of experiments essential Willingness and ability to work both independently and as part of a teamWorking Environment: Below is listed the working environment/requirements for this role:Able to communicate, receive, and understand information and ideas with diverse groups of people in a comprehensible and reasonable manner.Able to work upright and stationary and/or standing for typical working hours.Able to lift and move objects up to 25 poundsAble to work in non-traditional work environments.Able to use and learn standard office equipment and technology withproficiency.May have exposure to potentially hazardous elements, including infectious agents, typically found in healthcare or laboratory environments.Able to perform successfully under pressure while prioritizing and handling multiple projects or activities.Benef